Co-overexpression of AVP1, PP2A-C5, and AtCLCc in Arabidopsis thaliana greatly increases tolerance to salt and drought stresses

Abiotic stresses such as salinity and drought impose a severe constraint on global food production, posing serious challenge in agriculture. Stacking beneficial genes transgenic crops will likely improve crop yield under abiotic stress conditions. Previous studies showed that individually overexpressing the Arabidopsis vacuolar H+-pyrophosphatase gene AVP1, protein phosphatase 2 A catalytic subunit PP2A-C5, chloride channel AtCLCc contributed to enhanced salt tolerance overexpression of AVP1 alone could also tolerance. We hypothesized co-overexpressing AtCLCc, would combine benefits these three genes, leading further increase plants due potential synergism genes. Indeed, co-overexpression significantly improved single well combined stresses. The AVP1/PP2A-C5/AtCLCc displayed robust growth produced greater amount biomass viable seeds than wild-type saline This study demonstrates successful several well-chosen is an effective strategy achieve potentially lead higher regions world with soil low precipitation.